黄连配伍吴茱萸对大鼠红细胞膜稳定性的影响

Compatible Effects of Coptidis Rhizoma and Fructus Evodiae on Stability of Erythrocyte Membrane in Rats

目的:研究黄连及黄连配伍吴茱萸对大鼠红细胞渗透脆性及谷胱甘肽含量的影响。方法:SD大鼠40只随机分为空白对照组、小剂量黄连组、大剂量黄连组和大剂量黄连配伍吴茱萸组4组,每组10只。将小剂量黄连、大剂量黄连和大剂量黄连配伍吴茱萸的水煎液通过灌胃途径,应用于SD大鼠。取各组大鼠抗凝血观察其红细胞初溶血的盐水浓度差异及红细胞内谷胱甘肽含量的改变。结果:与空白对照组比较,大剂量黄连组始溶血盐水浓度显著升高(P<0.01),大鼠红细胞内谷胱甘肽含量下降(P<0.01),小剂量黄连组和大剂量黄连配伍吴茱萸组虽有升高始溶血盐水浓度、降低红细胞内谷胱甘肽含量的趋势,但差异无统计学意义(P>0.05)。结论:将黄连以常用剂量较长时间给药,对正常大鼠红细胞膜稳定性无明显影响,超常大剂量较长时间给药,可引起大鼠红细胞溶血;黄连与吴茱萸配伍,吴茱萸可有效拮抗较高浓度黄连对红细胞膜稳定性的影响。

Objective： To study the effect of eoptidis rhizoma, compatibility of coptidis rhizoma and fructus evodiae on osmotic fragility and GSH content in the rat＇s erythrocyte.by respectively. Methods：40 SD rats were randomly divided into blank control group,low dose coptidis rhizoma group,high dose coptidis rhizoma group and compatibility of large dose coptidis rhizoma and fructus evodiae group （n=10）. Rats were intragastrically administrated corresponding decoction for 15 d.Then the change of osmotic fragility and GSH content in erythrocyte were observed. Results：Compared with blank control group, osmotic fragility of erythrocytes was significant increased, GSH content was decreased in large dose coptidis rhizoma group（P〈 0.01 ）. Conclusions：Coptidis rhizoma has no effect on stability of erythroeyte membrane in normal rat administrated with general dosage for long time.The rats administrated coptidis rhizoma with exceeding dosage for long time showed erythroeyte hemolysis.Fructus evodiae can antagonize effect of high dose eoptidis rhizoma on stability of erythrocyte membrane.