苓桂术甘汤对急性心肌梗死心室重构模型大鼠TNF-α、IL-6和ET-1的影响

Effects of Lingguizhugan Decoction on TNF-α,IL-6 and ET-1 of Rats With Acute Myocardial Infarction

目的:观察苓桂术甘汤对急性心肌梗死心室重构模型大鼠血清TNF-α、IL-6和ET-1的影响,探讨其干预急性心肌梗死后心室重构的机制。方法:采用冠状动脉结扎法制备急性心肌梗死大鼠模型,造模2 w后将模型大鼠随机分为模型组、卡托普利组、苓桂术甘汤小(2.1 g生药/kg)、中(4.2 g生药/kg)、大(8.4 g生药/kg)剂量组,另设假手术组,分别灌胃给药,连续给药4 w,采用ELISA法检测各组大鼠血清TNF-a、IL-6和ET-1的含量。结果:与假手术组相比,模型组大鼠血清TNF-α、IL-6和ET-1含量显著升高(P<0.01);而苓桂术甘汤各剂量组能够显著降低模型大鼠血清TNF-α、IL-6和ET-1含量(P<0.01,P<0.05)。结论:苓桂术甘汤能够通过调节细胞因子网络干预急性心肌梗死后的心室重构。

Objective：To observe the effects of Lingguizhugan decoction on TNF-c~,IL-6 and ET-1 of rats with acute myocardial infarction （AMI） for 2 weeks, and explore the mechanism of Lingguizhugan decoction intervene ventricular remodeling of post-AMI. Method：AMI model was established by ligating coronary artery.The model rats were randomly divided into model group, captopril group, Lingguizhugan decoction low （2.1 g/kg）, middle （4.2 g/kg）and high （8.4 g/kg） dosage groups. 10 rats were selected as sham-operated group.Rats in sham-operated group and the other 5 groups have being administered medicine for 4 weeks constantly.The contents TNF-c~, IL-6 and ET-1 in serum of rats were detected by ELISA. Result：Compared with sham-operated group,contents of TNF-c~,IL-6 and ET-1 in model group were increased significantly （P〈0.01）. Compared with model group,contents of TNF-α,IL-6 and ET-I were decreased in Lingguizhugan decoction low, middle, high dosage groups and captopril group（P〈O.01, P〈0.05 ）. Conclusion：Lingguizhugan decoction can prevent ventricular remodeling through regulating cytokine networks..