尾加压素Ⅱ受体拮抗剂对阿霉素肾病大鼠中尾加压素Ⅱ影响的对比研究

Contrast Study of the Effect of Urotensin Ⅱ Receptor Antagonist on the Plasma Urotensin Ⅱ in Rats with Nephropathy

目的:观测尾加压素Ⅱ受体拮抗剂palosuran对阿霉素肾病大鼠尿中尾加压素Ⅱ(UrotensinⅡ;UⅡ)的影响,从而探究palosuran对微小病变肾病综合征(minimal change ne-phropathy syndrome;MCNS)的作用。方法:采用雄性Wistar大鼠80只,体重100±20g。随机选择其中10只做为健康组,其余70只大鼠尾静脉一次性注射阿霉素5mg/kg建立动物模型。注射后第7天检测大鼠24小时尿蛋白定量,若大鼠尿蛋白>30mg/24h者为MCNS模型成功,不合格者予以剔除。将合格的大鼠,随机分成2组,一组设为微小病变型肾病模型组,另一组在微小病变型肾病模型的基础上采用palosuran混入饲料中给药方法喂养,设为干预组。在实验第14、21、28天检测各组大鼠采用放射免疫法检测各组大鼠尿UⅡ的水平。结果:健康组大鼠尿UⅡ第14、21、28天检测值分别是1.46±0.18 ng/mL,1.52±0.12 ng/mL,1.54±0.43 ng/mL;模型组分别是2.49±0.11 ng/mL,4.48±0.19 ng/mL,6.56±0.27 ng/mL;干预组分别是1.95±0.1ng/mL,3.31±0.15 ng/mL,2.58±0.21 ng/mL。随着时间的延续,模型组大鼠尿UⅡ进行性升高(P<0.05),而palosuran对肾病大鼠尿UⅡ水平有明显影响,差异有显著统计意义(P<0.05)。结论:palosuran可能通过抑制UⅡ的作用而对微小病变肾病大鼠肾损害产生保护作用。

Objective：To observe the influence of Urotensin Ⅱ receptor antagonist（palosuran） to Urotensin Ⅱin urine of Adriamycin nephropathy rats,and to study the funcsion of palosuran to minimal change nephrotic syndrome（MCNS）.Methods：80 male Wistar rats weight 100±20g were enrolled in this study.10 of the rats were selected randomly only for the healthy group,and the remaining 70 rats were intravenously injected doxorubicin 5mg/kg establish animal models.On the 7 day after injection,to detect 24-hour urinary protein of the 70 rats.The MCNS model who is successful that the urine protein〉 30mg/24h.Then the failure to be removed.Qualified rats were randomly divided into two groups.One is the minimal change nephropathy group which named model group,another is the intervention group which based on the front to use palosuran mixed feed administration methods in.On the7th,14th and 28th day to determe the content of UⅡin urine of all rats were measured.Result：The entration of UⅡ of the healthy group on the 14,21,28days was（1.46±0.18 ng/mL）,（1.52±0.12 ng/mL）,（1.54±0.43 ng/mL）,（2.49±0.11 ng/mL）,（4.48±0.19 ng/mL）,（6.56±0.27 ng/mL） at the same time of the model group,and（1.95±0.1 ng/mL）,（3.31±0.15 ng/mL）,（2.58±0.21 ng/mL）of the intervention group.With the time going on,the urinary UⅡwas significantly increased in model group（P〈0.05）,and palosuran affected UⅡ in the rats with nephrapathy significantly（P〈0.05）.Conclusions：URA may protect renal damage on nephropathy by inhibiting the effect of UⅡ.