摘要
目的:合成木犀草素-Zn(Ⅱ)配合物,用两种分析方法考察其清除DPPH自由基的能力。方法:以无水乙醇为溶剂,在pH=9.76,温度为80℃时,木犀草素与醋酸锌作用合成木犀草素-Zn(Ⅱ)配合物;确定其结构后,将该化合物与DPPH作用,分别用高效液相色谱法和紫外分光光度法,测定其中DPPH的浓度,来考察该化合物对DPPH自由基的清除作用。结果:木犀草素-Zn(Ⅱ)配合物的组成为:C15H9O6Zn(COOCH3)(H2O).H2O;木犀草素-Zn(Ⅱ)配合物对DPPH自由基的清除作用强于配体木犀草素本身。结论:木犀草素与Zn2+进行络合后,增强了配体本身抗DPPH的生物活性。
Objective：Luteolin--Zn （Ⅱ） Complex was synthesized to study its activity oi removing DPPH radical by two analytical methods. Methods： Complex was prepared by reacting luteolin with Zn （Ac）2 in pH 9.76 ethanol solution in the temperature of 80℃. After confirming its molecular structure, DPPH free radical scavenging activity of the complex was investigated by measuring the concentration of DPPH free radical, re- spectively by HPLC and UV- spectrophotometer. Results： Composition of the complex was C15H906Zn （COOCH3） （H2O）·H20. DPPH free radical scavenging effect of luteolin-Zn （Ⅱ） complex was stronger than that of luteolin. Conclusion=DPPH free radical scavenging activity of luteolin was enhanced by compounding with Zn （Ⅱ）.
出处
《黑龙江医药科学》
2012年第4期16-18,共3页
Heilongjiang Medicine and Pharmacy
基金
佳木斯大学重点科研究项目
编号:12511576
