冰冻切片制作技术的改进及临床应用

The improvement and clinical application of frozen section production technology

目的:探讨冰冻切片制作过程中的影响因素及改进措施,提高冰冻切片的临床应用价值。方法:选取新鲜活体组织,取材后直接冻结,采用恒温式冰冻切片机切片,冰冻固定液固定,HE染色。结果:质量较好,染色鲜艳,显微镜下组织结构、细胞形态清晰,细胞核浆对比分明。结论:制作高质量的冰冻切片需要在取材、冷冻、切片、固定等多个环节上精益求精,不断改进,以提高病理诊断率,提高其临床应用价值。

Objective：To study the influence factors and improvement measures in the process of making cryostat sections,and to improve the value of clinical application of frozen section. Methods：Choose fresh and living tissue,directly freeze,and section by the use of constant temperature freezing microtome, and then fix in Stationary liquid,and finally HE stain. Results： Frozen sections have better quality and bright staining, with clear organization structure and cell morphology, and cell nucleus and cell plasma have Sharp contrast. Conclu- sion=If we want to produce high quality frozen sections, we need to refine on and make continuous improvement on multiple links which include material, freezing, cutting, fixing and so on, in order to improve the rate of pathological diagnosis and the value of clinical aonlication.