促红细胞生成素和甲基泼尼松龙对大鼠急性脊髓损伤的疗效比较

Comparison of curative effects between erythropoietin and methylprednisolone for acute spinal cord injured rats

目的探讨促红细胞生成素(EPO)和甲基泼尼松龙(MP)对大鼠急性脊髓损伤(ASCI)的疗效。方法按改良Allen′s撞击法制作急性不完全性脊髓损伤的大鼠模型。将60只雄性SD大鼠随机分为3组:EPO组、MP组和对照组,每组20只,在模型制作成功后8h、24h、3d、7d4个时间点取脊髓标本。比较各组在各个时间点上的运动功能评分、凋亡细胞计数、脊髓病理改变等指标。结果 EPO组和MP组在伤后2、8h的运动功能评分和伤后8、24h凋亡细胞计数比较,差异无统计学意义(P>0.05),但均优于对照组(P<0.01);在伤后3、7d时,EPO组的运动评分和凋亡细胞计数优于MP组(P<0.05),且均明显优于对照组(P<0.01)。结论 EPO比MP的抗凋亡作用更强、更持久,能显著减轻继发性脊髓损伤,促进运动功能恢复。

Objective To investigate the neuroprotective effect of erythropoietin（EPO） and methylprednisolone（MP） for rat acute spinal cord injury（ASCI）.Methods The incomplete ASCI rat model was established according to the improved Allen′s method.60 male rats were randomly divided into the EPO group,MP group and control group,20 cases in each group.The samples of spinal cord were taken at the time points of 8 h,24 h,3 d and 7 d after spinal cord injury.The motor function scores,count of apoptosis cells and the pathological change of spinal cord were compared among the three groups at corresponding time points.Results There was no statistical difference in the average scores at 2,8,24 h after injury,and the count of apoptosis cells at 8,24 h after injury between the EPO group and the MP group（P〉0.05）,but both of them were better than those in the control group（P〈0.01）.At 3,7d after injury,the motor function scores and count of apoptosis cells were superior to those in the MP group（P〈0.05） and significantly superior to those in the control group（P〈0.01）.Conclusion EPO has stronger and more persistent anti-apoptosis effect,can significantly alleviate secondary spinal cord injury and promote recovery of motor function.