贵州省2006-2010年脊髓灰质炎实验室监测与评价

Surveillance and valuation of Labortory for Poliomyelitis from 2006-2010 in Guizhou Province

目的分析贵州省脊髓灰质炎(脊灰)实验室监测数据,评估运转情况,为贵州省继续维持无脊灰状态提供实验室数据。方法采用病毒分离、鉴定与核苷酸序列分析的方法,对贵州省2006-2010年报告的急性弛缓性麻痹(AFP)病例、接触者及其他人群粪便标本进行病毒学检测和结果分析。结果 2006-2010年贵州省脊灰实验室共收到2 204份AFP病例粪便标本,332份AFP病例接触者粪便标本,310份其他人群粪便标本。从AFP病例中分离到非脊灰其他肠道病毒(NPEV)209株,脊灰病毒(PV)54株;从AFP病例接触者中分离到NPEV33株,PV6株;其他人群中分离到NPEV27株,PV9株。所有PV阳性分离物均送到国家脊灰实验室进行型内鉴定,全部为疫苗相关株。贵州省脊灰实验室每次均通过WHO和国家脊灰实验室的能力验证和现场认证评估。结论 2006-2010年贵州省脊灰实验室监测系统运转正常,未发现脊灰野病毒(WPV)或疫苗衍生脊灰病毒(VDPV)。贵州省继续保持无脊灰状态。

Objective To analyze the surveillance data in polio laboratory of Guizhou and evaluate the running status, provide laboratory data for maintaining polio-free sataus in Guizhou province. Methods Adopted the methods of virus isolation, virus identification and nucleotide sequencing to carry out the virological examination and made an analysis on the result of polio virus surveillance of AFP cases and their contacts by collecting stool specimens in 2006-2010 form whole province. Results A total of 2 204 part stool samples of AFP cases were collected during 2006-2010, 332 part stool samples of APF contacts and 310 part stool samples of other crowd were collected to polio laboratory of Guizhou province. 209 non-polio enterovirus （NPEV） strains were isolated from AFP cases, and 54 poliovirus strains were isolated from AFP cases. 33 NPEV strains were isolated from AFP contacts, and 6 poliovirus strains were isolated from AFP contacts. 27 NPEV strains were isolated from other crowd, and 9 poliovirus strains were isolated from other crowd. ALL of the polio positive isolates were identified by national polio laboratory. All of the polio viruses were vaccine-associated polio virus strains. The polio laboratory of Guizhou province passed the proficiency testing and site certification assessment of the WHO and National Polio Laboratory every time. Conclusion The polio laboratory in Guizhou province ran normal, no wild- type poliovirus （WPV） or Vaccine-derived poliovirus （VDPV） was found. Polio-free states were maintained in Guizhou province.