厌氧与光合微生物联合制氢工艺实验研究

Experimental Study of Hydrogen Production by Anaerobic and Photosynthetic Microorganisms Combined

利用厌氧细菌暗发酵产氢和光合细菌光发酵产氢的优势和互补协同作用而联合起来的两步法制氢,探讨不同底物浓度对厌氧发酵阶段产氢的影响、厌氧发酵时间对产氢发酵过程的影响;光合微生物发酵随发酵时间的产氢情况。结果表明,葡萄糖浓度对厌氧生物产氢有很大的影响,15 g/L的葡萄糖浓度有较好的产氢量。葡萄糖利用率和挥发性脂肪酸的总量随厌氧发酵时间的变化情况表明,在厌氧发酵阶段,以葡萄糖为底物,最佳的葡萄糖浓度为15 g/L。在37 h的葡萄糖利用率达到72.08%,挥发性脂肪酸总量达到9 326.3 mg/L,每克葡萄糖累计产氢量为182 mL。在厌氧发酵时间37 h时把厌氧发酵的产物移到光合发酵反应器,接种位于生长对数期的光合细菌群,调节培养液的pH值和加入光源进行光合产氢,88 h时每克葡萄糖累计产氢达到352 mL,两步联合制氢每克葡萄糖累计产氢量共可达到534 mL。

In this study, we use the two-step method to produce hydrogen by the anaerobic bacteria in dark fermentation and bacteria of photosynthetic fermentation, which have co-advantages and complementary synergy. The influences of different substrate concentrations at the anaerobic fermentation stage, the anaerobic fermentation time and photosynthetic microbial fermentation with fermentation time on hydrogen productin are investigated. The experiment showed that glucose concentration had a great influence on the production of anaerobic bio-hydrogen,e, g. , glucose concentration of 15 g/L has better hydrogen production. The glucose utilization and total volatile fatty acid with the change of anaerobic fermentation time showed that during anaerobic fermentation using glucose as the substrate,the optimal glucose concentration is 15 g/L. Glucose utilization can reach up to 72.08 % in 37 h, the total amount of volatie fatty acids reach up to 9 326.3 mg/L, and 182 mL/g of the cumulative hydrogen production can be obtained. After 37 h of anaerobic fermentation, the product of anaerobic fermentation is moved to photosynthetic fermentation reactor and inoculated with photosynthetic bacteria of growing logarithmic phase under the suitable pH value of the medium and the light sources for photosynthetic hydrogen production. 352 mL/g of the cumulative hydrogen production was obtained in 88 h. The total amount of hydrogen production reached 534 mL/g with this two-step method.