氟苯尼考残留的酶联免疫检测方法的研究

Detection of Florfenicol Residues by Enzyme-linked Immunosorbent Assay

对氟苯尼考进行结构改造制备半抗原及抗原,免疫家兔得到多克隆抗体。经测定,该抗体对氟苯尼考和氟苯尼考胺均有反应,且灵敏度较高,效价达到1∶480 000;在此基础上通过对各实验条件参数的优化建立了间接竞争酶联免疫检测方法,该方法检测限为0.5μg/kg,以20,50μg/kg进行空白样品添加实验,回收率为82.5%～96.0%,批间变异系数在4.1%～15.2%,可初步用于动物组织样本中氟苯尼考残留的检测.

The structure of florfenicol was transformed to prepare the hapten antigen and antigen, the polyclonal antibody was obtained by immunized rabbit. The determination of the antibody showed high sensitivity and have reactions on florfenicol and florfenieol amine, the titer was 1 ： 480 000. On this basis, the indirect competitive ELISA method was established through the optimization of the parameters. The de- tection limit of the established ELISA method was 0.5 μg/kg, the sample recovery was 82.5% to 96.0% by 20,50 μg / kg addition to the blank experiment, and the inter-assay coefficient ranged from 4. 1% to 15.2%. The method could be used for preliminary detection of florfenicol residues in animal tissue sam- ples.