内皮抑素30肽对SGC-7901细胞增殖活性的影响

Experimental study on the role of Peptide 30 Derived from Endostatin in the proliferation process of SGC-7901 cells

目的研究经RGD修饰的人内皮抑素(endostatin)N-端的30肽对胃癌SGC-7901细胞增殖能力的影响。方法人工合成人内皮抑素1～30位氨基酸(30肽,25～31序列由RGIR GAD改为RGDRGD)所对应的核苷酸序列,连接到质粒pTYB2中,再转化至大肠埃希菌BL21(DE3)中表达,用WST-1试剂检测30肽对SGC-7901细胞增殖活性的影响。结果 30肽能够有效抑制SGC-7901细胞的增殖,并呈时间剂量依赖性。结论 30肽能有效抑制SGC-7901细胞的增殖,其在临床胃癌的治疗上具有潜在的应用前景。

Objective The effect of a 30-amino-acid N-terminal peptide of endostatin（aa 1-30,peptide 30） modified by RGD on proliferation of SGC-7901 cells was studied.Methods The nucleotide sequence encoding amino acids 1-30 of endostatin（peptide 30,with amino acids 25-31 mutated from RGIRGAD to RGDRGD） was artificially synthesized and cloned into the plasmid pTYB2 and expressed in E.coli（DE3）.The proliferation of SGC-7901 was observed by WST-1 kit.Results SGC-7901 cell viability was significantly inhibited in a time-and-dose dependent response by peptide 30.Conclusions Peptide 30 effectively suppressed the proliferation of SGC-7901 cells.Peptide 30 can potentially be used to treat gastric carcinoma in clinic.