草莓CO同源基因FaCO-2的克隆及表达分析

Isolation and Express Analysis of FaCO-2,a CO Homologue from Strawberry

以草莓(Fragaria×ananassa Duch.)品种花姬为试材,通过PCR和RT-PCR方法克隆出草莓CO同源基因FaCO-2的CDS和DNA全长序列,在此基础上通过实时定量PCR的方法检测了其在草莓植株各组织和花器官中的表达。结果表明,该基因DNA序列长度为1 882 bp,含1个内含子,其CDS长度为1 146 bp,编码382个氨基酸,与其他物种的CO同源基因氨基酸序列有较高的同源性,生物信息学分析表明该蛋白分子量为42 021.69 D,等电点pI=5.49。实时定量RT-PCR结果表明,在不同的组织、花器官中,FaC0-2的表达量存在差异,在充分展开的较大叶片中的表达量最高;在4轮花器官中,只在萼片中表达,而其他花器官中不表达。

The CO homologue, FaCO-2,was isolated from strawberry （Fragaria x ananassa cv. Huaji） with the method of PCR and RT-PCR. Its express pattern in different tissues and organs was checked with qRT-PCR. The full length of its DNA sequence is 1 882 bp and its CDS is 1 146 bp in length. FaCO-2 codes a predicted protein of 382 amino acids. The amino acid identity compared with other CO homologues is highly conserved. The protein molecular weight was 42 021.69 Da with an isoelectric point （PI） of 5.49. The real-time RT-PCR result showed that FaCO-2 mainly transcripts differ in different tissues and floral organs. It was detected its highest expression in the expanded leaf. In the four flower organs, it was only detected in sepal, while no transcription was detected in other flower organs.