野生二粒小麦导入普通小麦抗白粉病基因的分子标记

Molecular Mapping of a Powdery Mildew Resistance Gene Transferred from Wild Emmer into Common Wheat

普通小麦的抗病品种(系)7K65对当前流行的小麦白粉菌E09生理小种具有较好的抗性,遗传分析表明,其抗性由一个显性基因控制,命名为Ml7K65。利用SSR,EST-STS标记对该基因进行检测,共筛选到7个与之连锁的标记位点,它们在遗传图谱上的顺序为:Xcfa2086-XEST83-XEST89-Xksum193-Xmag633-Xcfd50-Ml7K65,各位点之间的遗传距离依次为5.4,2.8,0.4,2.4,0.4,2.0 cM。其中,Xcfd50为离Ml7K65距离最近的标记,图距为2.0 cM,最远标记Xcfa2086与Ml7K65图距为13.4 cM。进一步利用中国春缺四体材料对该基因进行染色体定位,结果表明,Ml7K65基因位于小麦染色体2A上。

Wheat line 7K65 was resistant to Blumeria graminis f. sp. tritici Race E09. Genetic analysis showed that the resistance was controlled by a single dominant gene,named MlTK65. After screening SSR,EST-STS markers,we found 7 marker loci were linked to the powdery mildew resistance gene Ml7K65. The genetic map of gene Ml7k65 indicates the order of these marker loci is Xcfa2086-XEST83-XEST89-Xksum193-Xmag633-Xcfd50-Ml7K65. The genetic distances of each interval are 5.4,2.8,0.4,2.4,0.4,2.0 cM, respectively. Xcfd50 is the closest marker, the genetic distance is 2.0 cM,while Xcfa2086 is the most distant maker with the genetic distance of 13.4 cM. Using Chinese Spring nullisomic-tetrasomic lines, we physically located the markers linked to Ml7K65gene on wheat chromosome 2A.