基于线粒体16S rRNA基因亚洲小车蝗7个地理种群的遗传变异分析

Genetic Variation Analysis of Seven Geographic Populations of Oedaleus asiaticus B.Bienko from Inner Mongolia based on Mitochondrial 16S rRNA Gene Sequences

为了解内蒙古草原优势害虫亚洲小车蝗(Oedaleus asiaticus)的种群状况,采用线粒体DNA(mtDNA)16SrRNA基因测序技术对7个不同地理种群的亚洲小车蝗的种群结构和遗传变异进行研究。通过对亚洲小车蝗28个个体的线粒体16S rRNA基因进行测序,获得1个长度为289 bp的同源序列,通过编辑,剪切有267 bp的序列基可用于这28个个体的比较。在267 bp的序列中,A+T约占69.9%,A+T含量明显高于G+C含量。其中有20个变异位点,约占所测核苷酸总数的7.49%,密码子第3位点上的变异最多。共检测出18个单倍型。以斑腿蝗科的鼓翅皱膝蝗Angaracris barabensis和槌角蝗科的宽须蚁蝗Myrmeleotettix Palpalis作外群构建NJ和UPGMA分子系统树。聚类结果表明,亚洲小车蝗mtDNA 16S rRNA基因序列不同个体间存在一定的分歧,呈现平行分布,没有明显的地理分布簇群,遗传变异与地理距离无明显相关性。

In order to understand the population status of Oedaleus asiaticus, one of the most dominant grass- hoppers in inner Mongolia,we investigated the genetic structure and genetic variation of seven populations of O. asi- aticus using mtDNA sequence of 16S rRNA. Partial sequence of 289 bp was obtained through sequencing 16S rRNA gene of 28 individuals from seven geographical populations and 267 bp can be used to analysis through editing and cutting. In the sequences obtained, A ＋ T% was about 69.9% and A ＋ T % was significantly higher than G ＋ C%. Twenty polymorphic sites were detected in the 267 bp nucleotide,accounting for 7.49% ,and the most of variation are on the third position of the codon. 18 hapotypes were detected in 28 individuals. The molecular phylogenetic tree was constructed with NJ and UPGMA method using Angaracris barabensis and Myrmeleotettix Palpalis outgroup spe- cies. The phylogenetic trees suggested that different individuals of O. asiaticus from seven geographical populations were located in different clusters, the branches of clusters are parallel and there is no obvious clusters of geograph- ical populations. There is no significant correlation between genetic variation and geographic distance.