摘要
为了建立鸡脐带间充质干细胞(Umbilical cord mesenchymal stem cells,UCMSCs)体外培养体系并对其进行生物学特性研究。取11～15日龄鸡胚,脐带分离获得UCMSCs,在L-DMEM培养基中培养,观察其形态。通过传代、生长曲线和周期分析其增殖情况,并研究体外诱导分化潜能。结果表明,鸡UCMSCs可在体外扩增培养,目前传代至P30,表达CD29、CD44和CD71,并具有向胰岛β样细胞诱导分化的潜能。鸡脐带中可以分离出UCMSCs,能够在体外培养,并可诱导分化为胰岛β样细胞。
The aim was to study on the biological characteristics of chicken UCMSCs by isolating culture in vitro. UCMSCs was isolated from umbilical cord of 11 -15 day-old chicken embryos . The suitable culture medium for UCMSCs was L-DMEM. Then the morphology and the growing proliferation condition and the differentiation po- tential in vitro of the chicken UCMSCs were observed. The results showed that Chicken UCMSCs can be cultured in vitro and have been passed to 30 generation. The UCMSCs expressed CD29,CD44 and CDT1 ,results displayed that the UCMSCs had the differentiation potential to isletβ-cells. UCMSCs from chicken can proliferate rapidly in vitro and be induced to isletβ-cells.
出处
《华北农学报》
CSCD
北大核心
2012年第4期123-129,共7页
Acta Agriculturae Boreali-Sinica
基金
转基因生物新品种培育科技重大专项(2011ZX08009-003-006
2011ZX08012-002-06)
基本科研业务项目(2011cj-9)
国家家养动物种质资源平台项目(2012)
关键词
鸡
脐带间充质干细胞
分离培养
鉴定
诱导分化
Chicken
UCMSCs
Isolation and in vitro culture
Identification
Differentiation potential
