摘要
对牛输卵管上皮细胞进行了原代及传代体外纯化培养,并对输卵管上皮细胞的生物学特性通过普通光镜、扫描电镜、免疫组化、流式细胞技术进行观察、鉴定和检测等相关研究,建立了一种稳定的制备纯度较高的牛输卵管上皮细胞体外培养方法,为进一步研究胚胎共培养和核移植供体细胞奠定了基础。
In this experiment, bovine oviductal epithelial cell was successfully primary cultured, subcutured and purified in vitro.The biological characteristics of these cells were studied by using scanning electron microscope,immunohisto-chemical, the growth curve, flow cytometry image.A stable and highly purified culture in vitro method for bovine oviductal epithelial cell was stablished, which laid a foundation for the further study of embryo co-culture and bovine nuclear transfer.
出处
《中国草食动物科学》
CAS
2012年第5期16-20,共5页
China Herbivore Science
基金
湖北省科技厅"省基金依托基地项目"(2010CBBB01801)
国家现代农业产业(奶牛)技术体系岗位经费(CARS-37-04B)
关键词
牛输卵管上皮细胞
培养纯化
生物学特性
bovine oviductal epithelial cell
culture and purification
biological characteristic
