乳腺浸润性导管癌患者血清胸苷激酶1表达及其与临床病理特征的关系

Expression of serum TK1 and its relationship with clinical and pathological features in patients with mammary gland invasive ductal carcinoma

目的 探讨细胞质胸苷激酶1（TK1）在乳腺浸润性导管癌（乳腺导管癌）患者血清中的表达,及其在乳腺导管癌侵袭转移过程中的作用。方法 回顾性分析行手术治疗的60例乳腺导管癌患者的临床资料,选择健康体检者为对照组,采用化学发光结合斑点杂交技术检测其血清TK1水平。结果 乳腺癌组TK1阳性表达率为75.0 %,显著高于对照组的6.7%（χ^2=23.155,P〈0.05）。患者肿瘤直径≥5 cm、TNM分期Ⅲ～Ⅳ期、淋巴结转移组患者血清TK1阳性表达率显著高于肿瘤直径〈5 cm、TNM分期Ⅰ～Ⅱ期、无淋巴结转移组（P〈0.05）。而TK1阳性表达率与性别、分化程度无明显相关性（P〉0.05）。结论 血清TK1在乳腺导管癌中呈高表达,血清TK1水平与肿瘤大小、TNM分期、淋巴结转移有关,高水平的TK1表明乳腺导管癌的侵袭和进展。

Objective To investigate the expression of serum thymidine kinase-1（TK1） and its relationship with clinical and pathological features in patients with mammary gland invasive ductal carcinoma（MGIDC）,and the rde of TK1 in the invasion and progression of MGIDC.Methods The clinical data of 60 patients with MGIDC who underwent surgical treatment were retrospectively analyzed,the healthy subjects were considered as control group.The serum TK1 level was detected by chemiluminescence combined with dot blot hybridization.Results The TK1 positive expression rate of breast cancer group was 75.0%,compared with the the control group（6.7%） the difference between the two groups was significant（χ^2=23.155,P〈0.05）.The TK1 positive expression rate of the tumor diameter ≥5cm group,the TNM stage III to IV group and lymph node metastasis group was significantly higher than the tumor diameter〈5cm group,the TNM stage Ⅰ to Ⅱ group and without lymph node metastasis group,the difference was significant（P〈0.05）.The TK1 positive expression rate was not statistically significant between the different age group and degree of tumor differentiation group（P〉0.05）.Conclusion The serum TK1 is high expression in patients with MGIDC,the serum TK1 level is closely related to the tumor size,TNM stage and lymph node metastasis.The high expression of serum TK1 shows the invasion and progression of MGIDC.