促红细胞生成素对心肌缺血再灌注损伤的保护作用及其机制研究

The protective effect of erythropoietin on myocardial ischemia-reperfusion injury in rats and its mechanism

目的 观察促红细胞生成素（EPO）对心肌缺血再灌注损伤（MIRI）的保护作用及可能的机制。方法 60只雄性SD大鼠随机均分为三组：假手术组,缺血再灌注损伤（I/RI）组和EPO＋I/RI组。采用开胸结扎LAD 30 min、再灌注150 min的方法建立大鼠MIRI模型,模型制备前给予EPO腹腔注射5000 u/kg,其余两组大鼠腹腔注射等量0.9%氯化钠注射液。采用硫代巴比妥（TBA）法测定血清丙二醛（MDA）含量,采用酶连免疫吸附反应（ELISA）法测定心肌损伤标志物（cTnⅠ、CK、CK-MB）含量。结果 I/RI组大鼠血清MDA、MPO水平显著高于假手术组（t=10.445、9.848,均P〈0.05）;经EPO干预后,EPO＋I/RI组大鼠血清MDA、MPO水平显著低于I/RI组（t=5.087、6.683,均P〈0.05）。I/RI组大鼠血清cTnⅠ、CK 、CK-MB水平显著高于假手术组（t=8.153、5.411、3.729,均P〈0.05）;经EPO干预后,EPO＋I/RI组大鼠血清cTnⅠ、CK 、CK-MB水平显著低于I/RI组（t=4.808、4.089、3.002,均P〈0.05）。结论 EPO对MIRI有保护作用,其保护作用可能是通过其抗氧化、抗炎以及对心肌细胞的保护效应来实现的。

Objective To investigate the protective effect and possible mechanisms of erythropoietin（EPO） on myocardial ischemia-reperfusion injury（MIRI） in rats.Methods Ninety male SD rats were randomly divided into three groups,the sham operation group（n=20）,I/RI group（n=20）,and EPO treatment combined I/RI group（n=20）.The left anterior descending coronary artery was ligated for 30min utes and then loosed for 150min utes to establish the rat model of MIRI.Rats of EPO＋I/RI group were injected with EPO（5000u/kg） in abdominal cavity,the others were injected with nomal sodium.The serum MDA content was determined in a method of the thiobarbituric（TBA）,the cardiac injury markers（cTn Ⅰ,CK,CK-MB） contents were determined by ELISA.Results The serum MDA and MPO levels of the I/RI group were significantly higher than the sham group,difference was significant（t=10.445,9.848,all P〈0.05）;Intervention by EPO,the serum MDA and MPO levels of the EPO＋I/RI group were significantly lower than the I/RI group,the difference was significant（t=5.087,6.683,all P〈0.05）.The serum cTnⅠ,CK and CK-MB levels of the I/RI group were significantly higher than the sham group,the difference was significant（t=8.153,5.411,3.729,P〈0.05）;Intervention by EPO,the serum cTnⅠ,CK and CK-MB levels of the EPO＋I/RI group were significantly lower than the I/RI group,the difference was significant（t=4.808,4.089,3.002,all P〈0.05）.Conclusion EPO has protective effect on MIRI,the effect may be through EPO′s antioxidant,anti-inflammatory and protective effect on myocardial cells in the MIRI rats.