高速逆流色谱法分离大豆异黄酮中的大豆苷和染料木苷

Separation of Soybean Isoflavone monomers by high speed counter-current chromatography

大豆异黄酮对前列腺癌、乳腺癌、结肠癌等具有抑制肿瘤细胞增殖及抑制肿瘤生长作用.为了分离得到制备级的大豆异黄酮单体,实验采用高速逆流色谱法(HSCCC)分离纯化大豆异黄酮,并选用高效液相色谱法(HPLC)测定样品的质量分数.通过对溶剂体系的选择,以及对主机转速、流速和进样量的优化,确定了分离纯化大豆异黄酮单体的最佳工艺条件.在最佳工艺条件下,对大豆异黄酮进行分离纯化,分离得到大豆苷91.65%,染料木苷92.97%.

Soybean Isoflavanes have antiproliferative effects on prostate cancer, breast cancer and colon cancer and it can inhibit the growth of tumor. In order to get the monomers, HSCCC was used to separate daidzein and genistein from Soybean Isoflavanes. Through the selection of suitable solvent system and speed of revolution, and the optimization of flow rate and injection volume, we get the optimum condition. The two-phase solvent system was composed of acetic ether-acetic acid-water（5 ： 1 ： 10 in volume ratio）. The machine speed was 850 r/min, while the flow rate was 1.5 mL/min, and the injection amount was 12 mg. In this condition, the purities of daidzein and genistein respectively was 91.65% and 92.97%.