富硒与富锗桦褐孔菌多酚的体内外抗氧化作用

In vitro and in vivo Antioxidant Effects of Polyphenol in Selenium-enriched and Germanium-enriched Inonotus obliquus

研究桦褐孔菌多酚（IOP）以及富硒培养的桦褐孔菌多酚（Se-IOP）、富锗培养的桦褐孔菌多酚（Ge-IOP）的抗氧化作用。以试剂盒法测定抗活性氧单位、MDA值,分光光度法测定脂肪氧合酶活力、红细胞溶血抑制率、谷胱甘肽过氧化物酶活力。在化学模拟体系中,4.0mg/mL的IOP、Se-IOP与Ge-IOP的抗氧化活性单位分别为52.36、89.21、82.93U/mL,对脂肪氧合酶活性的抑制率分别为34.15%、42.22%、50.53%。在细胞与组织水平的体外抗氧化体系中,4.0mg/mLIOP对H2O2诱导的小鼠红细胞溶血抑制率为69.65%,而1~4.0mg/mLSe-IOP与Ge-IOP溶血抑制率均大于100%;与对照组比较,1~4.0mg/mLIOP、Se-IOP与Ge-IOP均可极显著地抑制小鼠肝匀浆自发性产生MDA（P〈0.01）。个体水平的体内抗氧化体系中,腹腔注射IOP、Se-IOP与Ge-IOP（4.0mg/mL,0.5mL/d,12天）时,肝组织的自发性MDA指标均极显著下降（P〈0.01）;腹腔注射1~4.0mg/mLSe-IOP与Ge-IOP后,各剂量组小鼠血清与肝匀浆谷胱甘肽过氧化物酶GSH-PX活力均极显著增强（P〈0.01）。IOP、Se-IOP与Ge-IOP在体内外均具有明显的抗氧化作用,富硒、富锗可增强桦褐孔菌多酚的抗氧化能力。

The aim was to investigate the antioxidant effects of Inonotus obliquus polyphenol （IOP）, Selenium-enriched Inonotus obliquus polyphenol （Se-IOP） and Germanium-enriched Inonotus obliquus polyphenol （Ge-IOP）. The reagent kit methods were used to determine anti-reactive oxygen species （anti-ROS） unit and malondialdehyde （MDA） value, the spectrophotometry methods were used to determine lipoxygenase activity, inhibitory rate of red blood cell （RBC） hemolysis and glutathione peroxidase （GSH-Px） activity, respectively. In chemical modified systems, the antioxidant activity of 4.00 mg/mL IOP, Se-IOP and Ge-IOP were 52.36 U/mL, 89.21 U/mL and 82.93 anti-ROS units, the inhibiting rates on lipoxygenase were 34.15%, 42.22% and 50.53%, respectively. In vitro cellular and tissular antioxidant systems, the inhibitory rate of 4.00 mg/mL IOP on mice RBC hemolysis by H2O2 inducement was 69.65%, which of 1-4.0 mg/mL Se-IOP and Ge-IOP were above 100%. The inhibitory effects of 1-4.0 mg/mL IOP, Se-IOP and Ge-IOP on the formation of MDA in mice without inducement were very significantly as compared with control group （P 0.01）. In vivo antioxiddant systems, the MDA value in mice liver decreased very significantly when the mice were intra-peritoneal injected with IOP, Se-IOP and Ge-IOP （4.0 mg/mL, 0.5 mL/d, 12 days） respectively （P0.01）, the GSH-Px activities in mice serum and liver also increased very significantly after being injected with Se-IOP and Ge-IOP （1-4.0 mg/mL, 0.5 mL/d, 12 days） respectively （P0.01）. IOP, Se-IOP and Ge-IOP had antioxidant effect in vitro and in vivo, Selenium and Germanium could increase the antioxidant activity of Inonotus obliquus polyphenol.