红肉火龙果果皮色素的纯化及稳定性的研究

Study on Purification and Stability of Pigment from Red-purple Pitaya (Hylocereus polarizes) Peel

为了研究大孔吸附树脂纯化火龙果果皮色素的条件和纯化后色素的稳定性,采用静态试验和动态试验确定纯化条件,计算纯化色素的色价,并观察纯化色素在不同条件下的稳定性。结果表明:在供试的5种大孔吸附树脂中,HPD100树脂的吸附和洗脱效果最好。室温下,以30%乙醇为洗脱剂,吸附流速和洗脱流速10mL/min时,纯化的红肉火龙果果皮色素色价为72.33,是未纯化前的5.39倍。光照不利于色素的稳定,Na+、K+、Mg2+、Ca2+、EDTA以及葡萄糖对色素的稳定性影响不大,而Sn2+、柠檬酸和酒石酸加快色素的降解。由此得出,大孔吸附树脂能有效分离纯化火龙果果皮色素,色素应避光保存,避免接触Sn2+、柠檬酸和酒石酸。

In order to using the macroporous adsorptive resins to study the purification condition of pigment from red pitaya peel and stability of the pigment after purification, By using static and dynamic experimental methods, the purification conditions were studied, the color scale of the purificatory pigment was calculated, and observe the stability of the purificatory pigment under different conditions. The results showed that, among the 5 macroporous resins tested, HPD100 resin was the best in adsorption effect and desorption effect. At room temperature, when 30% of ethanol was used as desorption solution, adsorption and desorption flow velocities were both 10 mL/min, the color scale of the purified pigment was 72.33, and it was 5.39 times higher compared with that before purification. Light was not conducive to the stability of the pigment, Na＋, K＋, Mg2＋, Ca2＋, EDTA and glucose had little effects on the stability of the pigment, however, Sn2 ＋ , citric acid and tartaric acid accelerated the degradation of the pigment. In a word, macroporous adsorptive resins could effectively separate and purificate the pigment from red pitaya peel, pigment should be stored in the dark, and avoid contact with Sn2＋, citric acid and tartaric acid.