NGF对大鼠胚胎中脑神经细胞体外增殖和分化的影响

The Effects of NGF on Proliferation and Differentiation of Rat Midbrain Nerve Cell in Vitro

目的:探讨神经生长因子(nerve growth factor,NGF)对大鼠胚胎中脑神经细胞体外增殖和分化的影响。方法:在体外分离培养大鼠胚胎中脑神经细胞的培养液中加入不同浓度(10、50、100、200ng/ml)的NGF,培养不同时间,以不加神经营养因子的细胞为对照组,通过MTT法检测细胞活性,神经元特异性烯醇化酶免疫细胞荧光技术鉴定神经细胞,光镜下形态学观察各组大鼠中脑神经细胞体外增殖和分化情况。结果:胚胎中脑神经细胞胞体增大、突起延长且有丰富的神经纤维连结成网络状,细胞集落数增加,显示出剂量-效应关系。结论:一定剂量的NGF能促进大鼠中脑神经细胞分化和增殖,增强其活性。

Objective： To compare the effects of nerve growth factor （NGF） on the proliferation and differentiation of rat midbrain nerve cell in vitro. Method： The culturemedium of midbrain nerve cell swas added NGF group or withoutany neurotropic factors （control- group）, The NGF different concentration for 10, 50, 100, 200 ng/ml, in different time to train nerve cells. The proliferation and differentiation of midbrain nerve cell were observed by convert microscope and MTT detection cell activity detected and the Neurons were identified by neuron-specific enolase immunofluorescence technic. Results： The cultured embryonic nerve cell body were aggrandized, the cell protuberance was prolonged, plenty of neuron fiber formed net work, the number of survival spinal cord neurons, which showed the doseeffect relation. Conclusion： Certain concentration of NGF（50.0-100.0ng/ml） can promote the differentiation and proliferation of embryonic nerve cell, and enhance the activity of the neurons.