干扰素-γ基因多态性与干扰素治疗慢性乙肝患者持久应答的关系

Relationship between genetic polymorphisms of interferon and sustained response of interferon in CHB patients

目的探讨干扰素-γ(IFN-γ)启动子基因874位点的单核苷酸多态性(SNP)与IFNα-2b治疗慢性乙肝(CHB)持久应答的关系。方法选择CHB患者106例,应用PCR-SSP技术分析宿主的IFN-γ启动子基因874位点的SNP,患者给予IFNα-2b治疗1年,随访1年,比较SNP与IFNα-2b疗程结束时完全应答、停药后随访1年完全应答(持久应答)的关系。结果在标准疗程结束时,完全应答的患者中三种基因型的分布无统计学差异(χ2=3.594 9,P=0.165 7)。而在持久应答患者中三种基因型的分布有统计学差异,TT基因型患者的持久应答率高于其他两种基因型患者(χ2=6.639 8,P=0.036 1)。结论 CHB患者对IFNα-2b治疗的持久应答与IFN-γ基因型有一定关联性,尤其与TT基因型关联更大。

Objective To study the possible relationship between genetic polymorphisms of interferon-γ/and sustained response of interferon in CHB patients. Methods Clinical data from 106 CHB patients in the original trial were analyzed. The SNP of INF-γ gene intron at position1 ＋ 874 was determined by polymerase chain reaction with sequence specific primers （PCR-SSP）. all patients received interferon treatment, one course of treatment was one year, and followed up one year. Results At the end of the course, there was no significant difference between three genetypes in the complete response patients（χ^2 =3.594 9 ,P =0. 165 7）. and at the end of the follow-up, there was significant difference between Lhree genetypes in the complete response （ sustained response, SR） patients, the SR rate of TT genetype patients was significantly higher than the other two genetype patients（χ^2 = 6. 639 8 ,P = 0.036 1 ）, Conclusion There was association between IFN- γ gene polymorphism and the SR of interferon treatment in CHB patients. This study suggested the possibility that IFN-γ, gene polymorphism（ intron 1 at position ＋ 874 ） might be important in determining an individual＇ s sustained etticacy of interferon, especially Tr genetypo.