微柱凝胶法交叉配血不合原因分析

The incompatibility reason analysis of microcolumn gel blood crossmatching test

目的探讨微柱凝胶法交叉配血不舍的原因并审j定相应的输血策略，以确保临床输血安全。方法采用微柱凝胶法进行交又配血试验，并对交叉配血不合的受血者、供血者血样进行血型血清学检查。结果21735例交叉配血试验；交叉配血不合193例；其中红细胞直抗阳性155例，占交叉配血不舍80．3％；不规则抗体阳性17例，占交叉配血不舍8．8％；自身抗体阳性14例。占交叉配血不合7．3％；其他原因7钢，占交叉配血不合3．6％。结论红细胞直抗、不规则抗体、自身抗体是引起微柱凝胶法交又配血不合的主要原因。

Objective The purpose of the article was to investigate the reasons of the incompatibility of Mircrncolumn gel blood cross- matching technology, and to take relevant measures to ensure the softy of blood transfusion. Methods Mircrocolumn gel technology was applied to analyze the blood crossmatehing of the incompatible blood supplier and recipient＇s blood samples, and blood group serology test of them were detected too. Results The rese.arch involved 21735 blond erossmatching trials, including 193 mismatch eases. In the 193 eases, there were 155 RBC antiglobulin positive cases, which accounted for 80.3% of the mismatch cases; 17 irregular antibodies positive cases, which accounted for 8.8% of the mismatch eases; 14 antoantibodies positive eases, which accounted for 7.3% of the mismatch cases; 7 cases of other rea- sons, which accounted for 3.6% of the mismatch cases. Conclusion The main causes of the mismatch of mircrocolumn gel blood crossmatehing were the RBC antiglobulin, the irregular antibodies and the autoantibodies.