负载超顺磁性四氧化三铁的抗体靶向聚合物胶束的构建

Load super paramagnetic ferroferric oxide antibody targeting of polymeric micellar construction

目的：构建一种新型纳米抗体靶向MRI造影剂，负载超顺磁性四氧化三铁（superparamagnetic iron oxide，SPIO）聚合物胶束。方法：采用两亲聚合物包覆法制备负载SPIO的聚合物胶束，并检测该聚合物胶束的粒径、形貌、顺磁性氧化铁的含量、磁化曲线；用免疫方法检测聚合物胶束连接抗体的情况、普鲁士蓝染色法观察铁在前列腺癌细胞中铁标记率。结果：合成的负载SPIO的抗体靶向聚合物胶束的粒径为38．1±0．4；靶向负载SPION的聚合物胶柬中SPIO的含量为35．8％，电镜下负载SPION的靶向聚合物胶束成球形，粒径小且每个胶束里只有一二个SPIO粒子；功能化后的负载SPIO的性能并没有减弱；通过免疫荧光法证实前列腺干细胞抗原（PSCA）抗体连接在聚合物胶束，能促进了前列腺癌细胞对负载SPIO的抗体靶向聚合物胶束的吸收。结论：成功合成PSCA抗体靶向功能的SPIO聚合物胶束，在体外均显示出较好的靶向效果，可通过进一步的研究探索作为一种活体应用的MR／探针来检测前列腺癌。

Objective ： to construct a new nano antibody targeted MRI contrast agents, load super paramagnetic ferroferfic oxide （ superpammagnetic iron oxide, SPIO ） polymer miceUes. Methods ： the two amphipbilic polymer coating method for preparing supported SPIO polymeric mleelles, and detection d the polymeric mlcelles, particle size, morphology, superparamagnetie iron oxide content, magnetization curve; detection by immune method of polymeric micelles connected antibody, Prussian blue staining observation Fe AR in prostate cancer cells labeled rate. Results： synthetic load SPIO antibody targeting of polymeric micellar particle diameter was 38. 1± 0.4 ; target load SPION polymeric micelles in the content of SPIO is 35.8% , under the electron load SPION targeting polymer mlcelle into a spherical shape, small size and each miceUe in only one or two of the SPIO particle ; function of load the performance of SPIO does not diminish ; by immunefluorescence confirmed prostate stem cell antigen （ PSCA ） antibody is attached in polymeric micelles, can promote prostate cancer cells to load SPIO antibody targeting polymer miceUe absorption. Conclusion： the successful synthesis of PSCA antibodies targeting function SPIO polymeric micelles, in vitro showed better targeting effect, through further study and exploration as an in rive applica- tion of MRI probes for the detection of prostate cancer.