RNA干扰及厄洛替尼阻断EGFR信号途径对A549细胞抗增殖的影响

Inhibitory effects of RNA interference and Erlotinib by blocking epidermal growth factor receptor pathway on the proliferation of A549 cells

目的研究RNA干扰(RNAi)及厄洛替尼阻断表皮生长因子受体(EGFR)信号途径对肺腺癌A549细胞增殖的影响。方法肺腺癌A549细胞分为对照组、RNAi组、厄洛替尼组。采用倒置相差显微镜观察EGFR信号阻断前后细胞形态的变化,RT-PCR检测EGFR mRNA表达,流式细胞术检测细胞凋亡,Western blot检测EGFR及Bcl-2、Bax表达。结果 RNAi阻断EGFR mRNA表达下调。与对照组相比,RNAi组及厄洛替尼组A549细胞凋亡增加,EGFR表达降低,Bcl-2水平表达降低,Bax蛋白表达增高,Bcl-2/Bax比值降低。结论阻断EGFR信号途径可促进肺腺癌A549细胞凋亡,其机制可能与降低Bcl-2/Bax比值水平相关。

Objective To explore the inhibitory effect of RNAi or Erlotinib by blocking epidermal growth factor receptor pathway on the proliferation of A549 cells.Methods We divided the A549 cells into the control group,the RNA interference group and the Erlotinib group.The inverted microscope was used to detect the morphological changes of A549 cells before and after blocking epidermal growth factor receptor pathway.RT-PCR was used to detect the expression of EGFR mRNA.The apoptosis rate of A549 cells was measured by flow cytometry.The expressions of EGFR,Bcl-2 and Bax were detected by Western blot.Results The expression of EGFR mRNA in the RNA interference group was down-regulated.Compared with those of the control group,the apoptosis rate of A549 cells and the expression of Bax were higher,the expression of EGFR,Bcl-2 and the ratio of Bcl-2/Bax were lower in the RNA interference group and the Erlotinib group.Conclusion Blocking EGFR pathway can promote the apoptosis of A549 cells.The mechanism may be related to the down-regulating of the ratio of Bcl-2/Bax.