SphK1与食管癌侵袭和转移的关系

Relationships between SphK1 and esophageal cancer invasion and metastasis

目的探讨鞘氨醇激酶1(SphKl)通路在食管癌侵袭和转移中的作用及临床意义。方法采用Real-timePCR和Western blot检测SphKl在食管癌组织中的表达,设计构建SphKl靶向shRNA质粒,建立SphKl稳定沉默的细胞系。MTT和Transwell法检测SphKl基因沉默对EC-1细胞增殖、侵袭的影响,明胶酶谱检测其对基质金属蛋白酶-9(MMP-9)和MMP-2分泌的影响。结果 SphKl的mRNA和蛋白质表达水平与侵袭能力明显相关。SphKl-siRNA能显著抑制EC-1细胞的增殖和侵袭,并能显著抑制EC-1细胞的MMP-9和MMP-2蛋白分泌。结论SphKl与食管癌侵袭和转移关系密切,转染靶向SphKl基因的siRNA序列能够抑制食管癌EC-1细胞的增殖和侵袭,其机制与抑制MMP-9和MMP-2蛋白分泌密切相关。

Objective To investigate the possible role of the sphingosine kinase pathway 1（SphKl） in the invasion and metastasis of esophageal cancer and its clinical significance.Methods Real-time PCR and Western blot were used to detect the SphKl expression in esophageal cancer tissues.The SphKl-targeted shRNA plasmid and SphKl-stable-silencing cell lines were established.MTT and Transwell were used to detect the cell proliferation and invasion.Gelatin zymography was used to detect MMP-9 and MMP-2 secretion.Results The SphKl mRNA and protein expression levels were significantly related to invasive ability.The SphKl-siRNA could significantly inhibit the proliferation and invasion of EC-1 cells.The SphKl-siRNA significantly inhibited the EC-1 cells invasion,MMP-9 and MMP-2 protein secretion.Conclusions SphKl is closely related to esophageal cancer invasion and metastasis.Transfection of siRNA sequences targeting SphKl gene can inhibit proliferation and invasion of human esophageal cancer EC-1 cells,and the mechanism is closely related to inhibited MMP-9 and MMP-2 protein secretion.