心房颤动患者心房组织SIRT1表达与心房纤维化的相关性

Relationship between SIRT1 expression in atrial tissue and atrial fibrosis in patients with atrial fibrillation

目的观察心房颤动患者心房组织Ⅲ类组蛋白去乙酰化酶1(SIRT1)的表达及其与心房纤维化的关系。方法选择38例行心脏手术的风湿性心脏病患者,按照是否合并房颤分为两组:持续性房颤组25例(AF组),窦性心律组13例(SR组),于心脏手术时切取小块右心耳组织,采用实时荧光定量PCR法检测SIRT1mRNA的表达量,采用免疫组化法检测SIRT1蛋白表达量,行心脏超声评价心房结构情况,采用masson染色评价心房纤维化程度,比较两组患者SIRT1表达及评价SIRT1表达与心房纤维化的关系。结果 AF组患者SIRT1mRNA表达量高于SR组(P<0.05),AF组SIRT1蛋白表达量高于SR组(P<0.05),AF组SIRT1mRNA及蛋白表达量与心房内径无相关性(左房:r=0.198,r=0.047;右房:r=0.027,r=0.096,P均>0.05),AF组SIRT1mRNA及蛋白表达量与心房胶原容积分数呈负相关(r=-0.643,r=-0.721,P<0.05)。结论房颤患者心房组织SIRT1表达增加,与心房纤维化呈负相关,提示SIRT1可通过抑制心房纤维化在房颤中起作用。

Objective To observe the expression of sirtuin 1（SIRT1）,one of the class III histone deacetylases in atrial tissue in patients with atrial fibrillation（AF） and clear the relationship between the expression of SIRT1 and atrial fibrosis.Methods 38 patients with rheumatic heart disease who underwent cardiac surgery were divided into two groups： the AF group（atrial fibrillation,n=25） and the SR group（sinus rhythm,n=13）.The right atrial appendage tissue was obtained during the heart valvular surgery.The expression of SIRT1 mRNA in atrial tissue was detected by real-time quantitative PCR and the expression of SIRT1 protein was detected by immunohistochemical assay.The atrial structure was measured by the transthoracic echocardiography and the extent of atrial fibrosis was identified by masson staining.Results Compared with that of the SR group,the expression of SIRT1 mRNA in atrial tissue was significantly higher in the AF group（P0.05）,the expression of SIRT1 protein was also significantly higher in the AF group（P0.05）.Spearman correlation indicated that the expression of SIRT1 had no correlation with the dimension of atrium（left atrium： r=0.198,r=0.047,right atrium： r=0.027,r=0.096,P0.05） in the AF group.Spearman correlation indicated that the expression of SIRT1 had significantly negative correlation with the amount of collagen volume fraction in the AF group（r=-0.643,r=-0.721,P0.05）.Conclusion The expression of SIRT1 is increased in patients with AF,which indicates SIRT1 may affect AF by means of inhibiting the process of atrial fibrosis.