滋补脾阴方药对大鼠脊髓损伤后脑源性神经营养因子表达的影响

Influence of zibu piyin recipe on expression of brain-derived neurotrophic factor after spinal cord injury in rats

目的探讨滋补脾阴方药(ZBPYR)对大鼠脊髓损伤(SCI)的治疗效果及对脑源性神经营养因子(BDNF)表达的影响。方法将清洁级Wistar大鼠90只随机分为假手术组、SCI组、ZBPYR组各30只。于伤后即刻、24、48、72、120 h处死动物,处死前采用联合行为评分法(CBS)检查大鼠神经恢复情况,并用免疫组化染色和逆转录聚合酶链反应(RT-PCR)方法,检测BDNF和BDNF mRNA的表达。结果除假手术组外,其他各组大鼠CBS评分均呈明显下降趋势。SCI、ZBPYR组,自伤后24 h起,各时间点间CBS评分差异显著(P<0.05),并且BDNF、BD-NF mRNA表达开始升高,于伤后120 h达峰值,ZBPYR组的表达量显著高于SCI组(P<0.05)。结论 ZBPYR可通过升高BDNF的表达水平促进大鼠SCI后神经功能的恢复。

Objective To study the effects of zibu piyin recipe（ZBPYR）on the spinal cord injury （SCI）and the influence on the expression of brain-derived neurotrophic factor（BDNF）. Methods Ninety adult Wistar rats were randomly divided into three groups： sham operated group, SCI group and ZBPYR group. The rats were killed at 0, 24, 48, 72 and 120h after the surgery. The combine behavioral score（ CBS ） was used to detect the recovery of the rats nervous sys- tem before being killed, and the expressions of BDNF mRNA and BDNF were detected with RT-PCR and immunohisto- chemistry assay at different time points. Results The CBS presented a remarkable decreasing trend in SCI and ZBPYR groups, and the difference between the two groups after 24h-post injury was statistically significant （ P 〈 0.05 ）. BDNF and BDNF mRNA expressions in SCI and ZBPYR groups were up regulated significantly after 24h-postinjury and peak- ed at 120h-post injury, and the difference between the two groups was statistically significant（ P 〈 0.05 ）. Conclusion ZBPYR could improve the neurons function through up regulating the expression of BDNF after SCI.