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娃儿藤碱类化合物DCB-3503对TNBS诱导溃疡性结肠炎的治疗作用 被引量：2

Therapeutic effect of tylophorine analog DCB-3503 on TNBS-induced ulcerative colitis
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摘要 目的探讨娃儿藤碱类化合物DCB-3503对三硝基苯磺酸(TNBS)诱导溃疡性结肠炎的治疗作用及可能的作用机制。方法选用C57BL/6野生型小鼠24只,分为对照(EtOH)组、实验(TNBS+DMSO)组、治疗(TNBS+DCB-3503)组。造模3 d后,观察各组间结肠长度、病理切片以及肠系膜淋巴结中IFN-γ+炎症性细胞和调节性T细胞(Treg)的差异。分离肠黏膜上皮细胞(IECs)总蛋白,Western blot检测Treg标志蛋白Foxp3的变化。结果治疗组损伤较实验组明显减轻,其肠系膜淋巴结中IFN-γ+细胞显著减少,Treg细胞显著增多,且IECs中Foxp3表达明显上调。结论在溃疡性结肠炎中,DCB-3503通过诱导T细胞分化为Foxp3+Treg细胞,从而抑制IFN-γ+炎症性细胞发挥作用,最终缓解肠道的炎症反应。 Objective To evaluate the protective effect of tylophorine DCB-3503 on trinitrobenzene sulfonic acid （ TNBS） induced ulcerative colitis and the possible molecular mechanism. Methods 24 C57BL/6 wild-type mice were di- vided into three groups ： EtOH group, TNBS ＋ DMSO group and TNBS ＋ DCB-3503 group. Three days after the model was set up, the mice were killed and colon length, histological section, patterns of IFN-γ inflammatory cells and Treg cells were examined. Besides, the expression of Foxp3 in IECs was detected using Western blot. Results Compared with TNBS ＋ DMSO group, DCB-3503 treatment group displayed remarkably improved clinical symptoms. Percentage of IFN-,,/~ inflammatory cells in mesenteric lymph node was significantly decreased in TNBS ＋ DCB-3503 group. Meanwhile the percentage of Treg cells in mesenteric lymph node and the expression of Foxp3 in intestinal epithelial cells （IECs） were significantly increased after DCB-3503 treatment. Conclusion The increase of Treg cells and de- crease of IFN-γ inflammatory cells in DCB-3503 treatment group suggest that DCB-3503 can ameliorate the ulcerative colitis induced by TNBS via the growing number of Foxp3 Treg cells and their suppression on IFN-γ, inflammatory cells.
作者 孟宪邑 张芸 吴震州
机构地区 南开大学生命科学学院
出处 《山东大学学报（医学版）》 CAS 北大核心 2012年第10期81-85,共5页 Journal of Shandong University:Health Sciences
关键词 溃疡性结肠炎 三硝基苯磺酸 IFN-γ TREG FOXP3 Ulcerative colitis Trinitrobenzene sulfonic acid IFN-γ Treg Foxp3
分类号 R574.62 [医药卫生—消化系统]
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参考文献21

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山东大学学报（医学版）
2012年 第10期

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