石墨烯修饰离子液体电极制备癌胚抗原免疫传感器

Immunosensor for Carcinoembryonic Antigen Based on Graphene Modified Carbon Ionic Liquid Electrode

以离子液体1-丁基-3甲基咪唑六氟磷酸盐为黏合剂制备了碳糊电极.利用石墨烯和金纳米修饰碳糊电极,通过金纳米的吸附能力将癌胚抗体修饰到电极表面制成免疫传感器,用于癌胚抗原的灵敏测定.利用循环伏安法对传感器进行了表征.结果表明,修饰电极显著地促进了电子在电极表面的传递速度,提高了方法的检测灵敏度.在优化条件下,峰电流变化值与癌胚抗原的浓度在0.5～5.0μg/L和5.0～120.0μg/L范围内呈良好的线性关系.信噪比等于3时,癌胚抗原的检出限为0.05μg/L.新方法被应用于人血清样品分析.

Carbon ionic liquid electrode（CILE） was fabricated by using an ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate as binder.Graphene and gold nanoparticles were used to modify CILE.An amperometric immunosensor for sensitive determination of carcinoembryonic antigen was constructed after anti-carcinoembryonic antigen was immobilized on gold nanoparticles.The fabricated immunosensor were characterized by cyclic voltammetry.The results showed that the modified electrode greatly improved the transfer rate of electron and therefore enhanced the sensitivity of the developed method.Under the optimal conditions,the linearity was obtained by plotting the peak current against the concentrations of carcinoembryonic antigen in the range of 0.5~5.0 μg/L and 5.0~120.0 μg/L with a detection limit of 0.05 μg/L（RSN = 3）.The developed method was applied in the analysis of carcinoembryonic antigen in human serum samples.