胆固醇酯转运蛋白TaqIB基因多态性与脑梗死关系

Relationship of Cholesterol ester Transfer Protein TaqIB Polymorphism with Cerebral Infarction

目的探讨胆固醇酯转运蛋白(CETP)TaqIB基因多态性与动脉粥样硬化性血栓性脑梗死之间的关系。方法对220例脑梗死患者和220例健康者进行研究。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定所有研究对象血标本的CETP TaqIB基因多态性,通过对脑梗死组和对照组的CETP TaqIB基因多态性比较,对比分析。结果脑梗死组B1B1、B1B2、B2B2基因型比例分别是59.09%、32.73%、8.18%,对照组分别为47.27%、39.09%、13.64%,两者比较差异有统计学意义。B1、B2等位基因在脑梗死组的频率分别为75.45%、24.55%,在对照组为66.36%、33.64%,两者差异也有统计学意义。结论胆固醇酯转运蛋白TaqIB基因多态性可能与动脉粥样硬化性血栓性脑梗死的发生有关。其中B1等位基因可能是易感基因,而B2基因可能是保护基因。

Objective To explore the relation ship between the genetic polymorphism cholesterol ester transfer protein （CETP） TaqIB and atherosclerotic thrombotic cerebral infarction. Methods A total of 220 cases of cerebral infarction and 220 heahhy subjects were studied. Polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP） method was adopt to determine CETP TaqIB gene polymorphism for all specimens. CETP TaqIB gene polymorphism in cerebral infarction group was compared with control group. Results Atotal of The proportions of B1 B1, B1B2, B2B2 genotype in cerebral infarction group were 59.09% , 32.73% , 8.18% respec- tively, and were 47.27% , 39.09% , 13.64% in the control group. The difference had significant difference. The frequencies of B1, B2 allele in cerebral infarction group were 75.45% ,24.55% respectively, and were 66.36% ,33.64% in the control group, and the differ- ence also had significant difference in comparison. Conclusion CETP TaqIB genetic polymorphism may he associated with the occurrence of atherosclerotic thrombotic cerebral infarction. BI allele may be the susceptibility gene, but B2 allele may be the protective gene.