3110009F21Rik基因在小鼠胚胎发育中的表达研究

Expression Pattern of 3110009F21Rik Gene During Mouse Embryon ic Development

目的：探讨小鼠胚胎发育过程中3110009F21Rik基因的时空特异性表达模式,为后续功能研究奠定基础。方法：对E15.5小鼠胚胎脑组织进行印记分析,检测基因的印记表达状态;应用全胚胎和组织原位杂交技术检测3110009F21Rik基因在E9.5~E15.5小鼠胚胎中的特异性时空表达模式。结果：印记分析显示3110009F21Rik基因在E15.5脑组织中为父母本等位基因双表达;原位杂交结果显示3110009F21Rik基因在E9.5~E15.5脑组织中持续表达,在E9.5~E11.5主要脏器中未检测到,但自E12.5开始在主要脏器中持续表达,随着发育进程进行,目的基因在胚胎骨骼中的相对表达水平逐步升高,至E15.5阶段大部分骨骼中都检测到目的基因表达。结论：3110009F21Rik基因在脑组织中的持续表达和表达模式的动态变化提示其可能参与胚胎发育过程中大脑神经网络的构建,其在软骨原基和软骨中的相对表达逐渐增强表明其可能参与了小鼠胚胎过程中骨的发育和形成及软骨分化。

Objective： To investigate the developmental-specific expression pattern of 3110009F21Rik gene dur ing mouse embryogenesis.Methods： We detected the imprinting state of 3110009F21Rik gene by imprinting analy sis,and analyzed the expression pattern of 3110009F21Rik gene during E9.5~E15.5 stages through in situ hybrid ization.Results： Imprinting analysis showed that 3110009F21Rik gene was a di-allele expression gene;in situ hy bridization analysis verified that 3110009F21Rik gene showed persistent expression on the mouse embryonic brain from E9.5 to E15.5,and no expression signal was found in the main internal organs from E9.5 to E11.5,but we can detect a weak expression signal in the main internal organs from E12.5 to E15.5.With the development of the mouse embryonic,the expression of 3110009F21Rik gene on the cartilage was detected from E12.5 to E15.5 with a relative high expression in E15.5.Conclusion： The 3110009F21Rik shows a dynamic expression during mouse embryogenesis and may have an important role in the mouse embryo development especially in the neural network estimation and the cartilage formation.