光合细菌捕光蛋白基因pucBA的保守性研究

Conservation of pucBA genes encoding light-harvesting proteins in photosynthetic bacteria

为了验证光合细菌捕光蛋白基因pucBA的保守性,通过克隆嗜硫小红卵菌的捕光蛋白基因pucBA,连入到表达载体pRK415中,通过接合转移的方法把此基因导入到捕光蛋白基因缺失的类球红细菌菌株DD13中,通过双抗生素筛选,获得转化子,通过诱导培养,转化子的菌液颜色观察和捕光蛋白复合物的光谱分析证明,此转化菌株能够形成捕光蛋白复合物,上述研究表明的捕光蛋白基因pucBA属于进化过程中比较保守的基因。

In order to investigate the conservation ofpucBA genes, which encode light-harvesting proteins in photosynthetic bacteria, pucBA locus of Rhodovulum sulfidophilum was amplified by PCR and subcloned into expression vector pRK415. Then by conjugation, pucBA was introduced into mutant strains of Rhodobacter sphaeroides DD 13, whose light-harvesting protein genes were deleted. Many transformants were isolated through double-antibiotic screening. Through the color of culture broth and spectrum analysis of the light-harvesting complexes, it was found that the transformant strains acquired the ability to synthesize light-harvesting protein complexes by the induction expression ofpucBA. The results indicated that pucBA are evolutionarily conserved..