酿酒葡萄中内生酵母菌的分离与初步鉴定

Isolation and primary identification of endophytic yeast from wine grape

采用组织块培养法,从优质酿酒葡萄品种"赤霞珠"中分离出12株内生酵母菌,其中春季4株,夏季3株,秋季5株;从酿酒葡萄的不同组织部位均分离到了内生酵母菌,其中根部6株、茎部2株、叶部1株、果实3株。经形态、培养特征、子囊孢子和假菌丝的观察及生理生化实验,初步鉴定出这些菌株分别为瓶形酵母和克勒克酵母,其中瓶形酵母6株,克勒克酵母6株。

Twelve endophytic yeasts were isolated sparately from roots, stems and leaves of Xinjiang wine grape by tissue isolation method, in which, four strains were isolated in spring, three strains in summer and five strains in autumn. Among several parts of grape, six strains were seperated from root, two strains from stem, one from leaf and three from fruit. Through morphological appraisal, cultural characteristics, yeast pseudohypha observation, physiological and biochemical determination, these endophytic yeasts were identified as Pityros purom and KIeckera, six strains were Pityros purom, and six were Kleckera.