氮离子注入赤藓糖醇生产菌诱变筛选高产菌株

Screening of the high yield erythritol-producing strain by implantation with N^+ ion

为了获得赤藓糖醇高产菌株,以酿酒酵母NJWGYH30566为研究对象,利用N+注入进行诱变。注入N+的能量为15keV,剂量为20×1014cm-2、40×1014cm-2、60×1014cm-2、80×1014cm-2、100×1014cm-2、120×1014cm-2。结果表明最佳注入剂量为80×1014cm-2,选育出一株赤藓糖醇高产菌株H-32,赤藓糖醇产量提高17.52%,且副产物甘油降低26.9%,经连续10代遗传稳定性试验考察,其高产性能稳定,极大提高了生产效率。

N＋ ion implantation was used to mutate Saccharomyces cerevisiae NJWGYH30566 for obtaining higher-yield erythritol strain. The original strain was mutated by 15keV N＋ ion implantation with the dose of 20×10^14cm^-2、40×10^14cm^-2、60×10^14cm^-2、80×10^14cm^-2、100×10^14cm^-2 and 120×10^14cm^-2, respectively. Results showed that the optimal N＋ ion dose was 80×10^14cm^-2, and a strain of high-yield erythritol was obtained and named as H-32. Erythritol yield of the mutant was increased by 17.52%, and by-product glycerol was decreased by 26.9% compared to those of the original strain. Subculture results showed that the mutant strains had high genetic stability.