微波提取-高效液相色谱法同时测定月饼中的5种防腐剂和甜味剂

Simultaneous determination of five kinds of preservatives and sweeteners in moon cakes by microwave extraction-high performance liquid chromatography

该研究建立了微波提取-高效液相色谱法同时测定月饼中的5种防腐剂和甜味剂的分析方法,研究了流动相和检测波长,设计正交试验优化了微波提取的条件,并对比了微波提取和超声提取的效果。结果显示微波提取在提取效率上更有优势,提取时间只需要超声提取时间的1/15。该分析方法检出限为0.2mg/kg～0.70mg/kg,线性范围为0.4mg/L～400mg/L,加标回收率为96.0%～103.2%,满足分析检测的要求。

A method of simultaneous determination of 5 preservatives and sweeteners in mooncakes by microwave extraction-high performance liquid chromatography was set up. In this paper, mobile phase and detection wavelength were researched. Orthogonal experiment was carried out to op＇ti- mize the conditions for microwave extraction. Then extraction effect by microwave extraction was compared to the one by ultrasonic extraction. The result showed that microwave extraction was more efficient and it costed only 1/15 of the time ultrasonic extraction need. The analytical method showed that the limits of detection were 0.2mg/kg--0.70mg/kg, the linear ranges were 0.4mg/D-400mg/L and the recoveries in spiked samples were 96.0%-103.2%, which satisfied the need of analytical detection.