山芝烯二醇对体外培养成骨细胞成骨活性的影响

Effects of Serratenediol on the Osteogenic Activity of Cultured Osteoblasts in vitro

为研究玉柏石松提取物山芝烯二醇对体外培养小鼠颅骨成骨细胞活性的影响,采用MTT法、碱性磷酸酶(ALP)试剂盒、荧光定量PCR检测不同浓度药物作用不同时间后成骨细胞增殖、ALP活性和成骨活性相关基因,如原癌基因(c-jun、c-fos)、成骨转录因子(Osterix)、碱性磷酸酶(ALP)、Ⅰ型胶原(Col-Ⅰ)、骨钙素(OC)表达.结果显示:山芝烯二醇处理早期(3 d)促进成骨细胞增殖,促进c-jun、c-fos基因表达,先抑制后促进Osterix基因表达;晚期(9 d)促进ALP活性和ALP、Col-Ⅰ基因表达,对OC基因无明显影响.说明山芝烯二醇可以促进成骨细胞的增值,ALP活性及部分骨相关基因表达,是玉柏石松促进骨愈合的有效成分之一.

To study the effect of serratenediol（an extract from Lycopodium obscurum L.） on the osteogenic activity of cultured osteoblasts,MTT assay、 alkaline phosphate（ALP） assay kit and real-time PCR were used to determine the proliferation rate,ALP activity and osteogenic genes（c-jun、c-fos、Osterix、ALP、Col-Ⅰ、OC） expression of osteoblasts.The results elucidated that treatment of serratenediol for 3 days could promote the proliferation rate,the expression of c-jun and c-fos genes and first inhibit then enhance Osterix gene of osteoblasts.Serratencdiol treatment for 9 days could enhance the expression of ALP and Col-Ⅰ genes.There was no obvious effect of serratenediol on the expression of OC.So serratenediol could promote the proliferation rate,ALP activity and some osteogenic gene expression of osteoblasts.It is one of the effective component from Lycopodium obscurum L.for bone healing.