西北地区东片部队医疗机构物表细菌抗消毒剂基因qacEΔ1检测研究

Detection on Disinfectant-resistant Gene of qacEΔ1 in Bacteria on the Object Surface of Military Medical Institutions in East Districts of Northwest China

目的研究西北地区东片部队医疗机构物体表面细菌抗消毒剂基因qacEΔ1的阳性率。方法用PCR方法检测物体表面分离的细菌的qacEΔ1基因。结果 167株细菌中有69株细菌检出了抗消毒剂基因,总阳性率为41.3%。蜡样芽孢杆菌、白色念珠菌、鲍曼不动杆菌中未检出qacEΔ1基因。在阳性菌株中,大肠杆菌的阳性率最高,为60.4%。结论西北地区东片部队医疗机构物体表面细菌抗消毒剂基因qacEΔ1阳性率较高,在消毒中不应广泛使用季铵盐类消毒剂。

Objective To analyze the positive rate of disinfectant - resistant gene of qacEA1 in Bactria on the object surface of military medical institutions in east districts of northwest China. Methods Gene of qacEΔ1 was detected by PCR. Results Gene of qacEΔ1 was found in 69 strains of samples. The total positive rate was 41.3%. qacEΔ1 was not found in B. anthracis, C. albicans, A. banimannii, or M. chelonae. The positive rate of qacEΔ1 in E. coli, which reached 60. 4%, was the highest among all tested positive strains of bacteria. Conclusion The positive rate of the gene of qacEΔ1 in bacteria from object surface in military medical institutions of east districts of Northwest China was comparatively high. Quaternary ammonium salt should not be used in disinfection.