中药复方对酒精性肝损害早期阶段阻断作用的实验研究

Blocking effect of Chinese herbal compound on alcoholic liver damage in early stages

目的:观察中药复方对酒精性肝损害早期阶段阻断作用。方法:将纯系雄性Wistar大鼠50只按体质量随机均分为正常组、模型7d组、模型30d组、中药7d组、中药30d组4组。以正常组和模型组为对照,采用白酒灌胃造模,同时给予中药复方,剂量为21g.kg-1.d-1,12周后,各组大鼠肝脏取材,应用光镜观察大鼠肝脏组织;测定各组大鼠乙醇脱氢酶(ADH)、谷胱甘肽硫转移酶(GST)活性水平及还原型辅酶Ⅰ(NADH)/氧化型辅酶(NAD+);测定各组大鼠肝功能指标。结果:各组大鼠ADH、GST活性水平及NADH/NAD+的测定结果显示,模型7d组与模型30d组较正常组均显著降低(P<0.05),各中药组无明显降低,与同期模型组相比有显著性差异(P<0.05);NADH\NAD+模型组较正常组均显著升高(P<0.05),各中药组无明显升高,与各模型组相比有显著性差异(P<0.05)。组织形态学各模型组肝组织损伤严重,各中药组肝组织损伤显著减轻。肝功能各中药组明显优于各模型组。结论:该中药复方较为有效地阻断了酒精对肝脏的损害。

Objective： To observe the blocking effect of Chinese herbal compound on alcoholic liver damage in early stages. Methods： 50 male rats were randomly divided into normal group, 7d model group, 30d model group, 7d herbal treatment group and 30d herbal treatment group. The normal group and model group as control, the model group and treatment group were garaged by liquor, at the same time the herbal treatment groups were given herbal compound, a dose of 21g·kg^-1·d^-1. After 12 weeks, all liver tissues of mice were taken out and observant by microscopic; ADH, GST, NADH/NAD^＋ and function of liver index were determined.Results： the measurement results of ADH, GST showed that 7d model group and 30d model group were significantly reduced than normal group （P〈0.05）, treatment groups were a little reducing, which showed significantly difference with model groups （P〈0.05）; the measurement results of NADH/NAD^＋ model showed, model groups increased significantly compared with normal group （P〈0.05）, treatment groups were a little rising, which showed significantly difference with model groups （P〈0.05）. Histomorphology present that the injury of liver tissue of model groups is more serious than treatment groups. Treatment groups of liver function were significantly better than the model groups. Conclusion： the traditional Chinese medicine compound effectively blocked on alcoholic liver damage.