摘要
[Objective] The technology of using c-amylase and glucoamylase to prepare microporeus potato starch was studied.[ Method ] Taking potato starch as raw materials, starch hydrolysis rate and the oil absorption as a measure of index, the influences of the reaction temperature, two enzymes proportion, the quantity of enzyme, the chroma of substrate, buffer solution pH and reaction time on microporous potato starch were inves- tigated. [ Result] The experimental results showed that the best technological conditions were reaction temperature 45 ℃, enzyme ratio （ glucoamy- lase/α-amylase）6, the quantity of enzyme （amount of enzyme and starch quality than） is 1.0%, the substrate quantity chroma of 0.14 g/ml, buffer solution pH 4, the reaction time 8 h. In such process condition, the oil adsorption rate of hydrolyzed potato starch was as high as 70.2%, starch hydrolytic ratio was 34.16%. [ Condmion] The study provided a basis for the development and utilization of microporous starch. Key words Microporous starch; Hydrolysis rate; Oil absorption rate; Preparation; Complex enzyme method; China
[Objective] The technology of using α-amylase and glucoamylase to prepare microporous potato starch was studied.[Method] Taking potato starch as raw materials,starch hydrolysis rate and the oil absorption as a measure of index,the influences of the reaction temperature,two enzymes proportion,the quantity of enzyme,the chroma of substrate,buffer solution pH and reaction time on microporous potato starch were investigated.[Result] The experimental results showed that the best technological conditions were reaction temperature 45 ℃,enzyme ratio(glucoamylase /α-amylase) 6,the quantity of enzyme(amount of enzyme and starch quality than) is 1.0%,the substrate quantity chroma of 0.14 g/ml,buffer solution pH 4,the reaction time 8 h.In such process condition,the oil adsorption rate of hydrolyzed potato starch was as high as 70.2%,starch hydrolytic ratio was 34.16%.[Conclusion] The study provided a basis for the development and utilization of microporous starch.
