碘量法快速测定乳和乳制品中的β-内酰胺酶

Determination of β-lactamase in milk and milk product by iodometric method

目的:建立了快速筛查乳与乳制品中的β-内酰胺酶的碘量法。方法:检测β-内酰胺酶分解β-内酰胺类抗生素所生成的青霉噻唑酸,间接判断牛奶中是否有β-内酰胺酶。结果:采用碘量法对牛奶中的β-内酰胺酶定性、半定量分析,结果表明,当酶浓度大于20 U/ml时,可以用直接碘量法对其进行定性分析。结论:本方法操作简单、分析时间短、重现性好,适用于乳与乳制品中β-内酰胺酶的间接快速检测。

Objective：An iodometric method was developed to detect β-lactamase in milk and milk product.Methods：It can make indirect judgement whether it has β-lactamase in the milk and milk product with detecting the decomposed product penicilloic acid.Results：The iodometric method can be employed for qualitative and semi-quantitative analysis of β-lactamase when the concentration of β-lactamase was more than 20 U/ml.Conclusion：The method was proved to be simple,rapid,reliable and suitable with good reproducibility for the determination of β-lactamase in milk and milk product.