高效液相色谱法同时测定婴幼儿食品和乳品中的叶黄素、α-胡萝卜素和β-胡萝卜素

Simultaneous determination of lutein,α-caroten and β-carotene levels in foods for infants and young children and milk products by HPLC

目的:建立高效液相色谱法同时测定奶粉中的叶黄素(Lut)、α-胡萝卜素(α-C)和β-胡萝卜素(β-C)的测定方法。方法:样品经皂化、石油醚-乙醚(1∶1)提取、水洗和浓缩后,以含2%水的甲醇-乙腈混合液(25∶75)和叔丁基甲基醚为流动相,在Waters C30柱中分离,二极管阵列检测器检测,外标法定量。结果:上述3种胡萝卜素在0.05μg/ml～4μg/ml范围内有良好的线性,相关系数R2均大于0.998,三水平加标平均回收率在80%～104%之间,每个加标水平的6次重复试验RSD<7.7%。结论:该方法可同时测定奶粉中的叶黄素、α-胡萝卜素和β-胡萝卜素含量,方法简便、快速、准确。

Objective ：A method for simultaneous determination of lutein, α - carotene and β- carotene levels in powdered milk by HPLC was developed. Methods： The homogenized samples were saponified and then extracted with petroleum ether and diethyl ether followed by wash and concentration. The mobile phase was methanol - aceto- nitrile mixture containing 2% water and methyl - t - butyl - ether. Carotenoids were separated by a C30 column and detected with diode array detector. An external standard method was used for quantification. Results： The linearities for three carotenoids were good at concentration ranges of 0. 05 μg/ml-4μg, the correlation coefficients were greater than 0. 998. The average spiked recoveries for three addition levels were from 80% to 104%. The relative standard deviation of each spiked test was less than 7.7%. Conclusion： The method can simultaneously determine lutein, α -carotene and β -carotene levels in powdered milk with simpleness, speediness and accuracy.