摘要
目的:建立尿中碘化物的液-液萃取毛细管气相色谱测定方法。方法:尿碘经衍生成易气化衍生物,通过有机溶剂萃取、离心,直接吸取有机层进样,经气相色谱分离,电子捕获检测器定量检测。结果:该法分离效果好,检出限为0.05μg/L,加标回收率为95.2%～98.6%,相对标准偏差(RSD)为1.0%～2.5%。结论:该方法简单,灵敏度高,可用于尿样中碘的测定。
Objective：To establish a method for the determination of iodide in urine by capillary gas chromatography after liquid-liquid extraction.Methods： The iodine in the sample was derived into easy-gasified derivatives.Then it was extracted with organic solvent and centrifuged.The extractant was directly injected into gas chromatography for analysis and quantified by electronic capture detector（ECD）.Results： Separation of the method was good and the detection limit was 0.05 μg/L with the recovery rate of 95.2%~98.6% and the relative standard deviation（RSD） was from 1.0% to 2.5%.Conclusion： This method is simple and sensitive and suitable for the detection of urinary iodine.
出处
《中国卫生检验杂志》
北大核心
2012年第9期2066-2067,2070,共3页
Chinese Journal of Health Laboratory Technology
关键词
液-液微萃取
尿碘
衍生
Liquid-Liquid Micro-extraction
Urinary iodine
Derivative
