摘要
目的:建立尿中碘化物的液-液萃取毛细管气相色谱测定方法。方法:尿碘经衍生成易气化衍生物,通过有机溶剂萃取、离心,直接吸取有机层进样,经气相色谱分离,电子捕获检测器定量检测。结果:该法分离效果好,检出限为0.05μg/L,加标回收率为95.2%~98.6%,相对标准偏差(RSD)为1.0%~2.5%。结论:该方法简单,灵敏度高,可用于尿样中碘的测定。
Objective:To establish a method for the determination of iodide in urine by capillary gas chromatography after liquid-liquid extraction.Methods: The iodine in the sample was derived into easy-gasified derivatives.Then it was extracted with organic solvent and centrifuged.The extractant was directly injected into gas chromatography for analysis and quantified by electronic capture detector(ECD).Results: Separation of the method was good and the detection limit was 0.05 μg/L with the recovery rate of 95.2%~98.6% and the relative standard deviation(RSD) was from 1.0% to 2.5%.Conclusion: This method is simple and sensitive and suitable for the detection of urinary iodine.
出处
《中国卫生检验杂志》
北大核心
2012年第9期2066-2067,2070,共3页
Chinese Journal of Health Laboratory Technology
关键词
液-液微萃取
尿碘
衍生
Liquid-Liquid Micro-extraction
Urinary iodine
Derivative