阴香植物精油对白色念珠菌生物膜的抑制研究

STUDY ON THE INHIBITORY EFFECTS OF ESSENTIAL OILS ON Candida albicans BIOFILM

目的观察阴香精油对白色念珠菌生物膜(BF)的抑制作用。方法采用改良Brown平板连续培养法制备生物膜和应用悬液定量杀菌试验法,对阴香精油抑制白色念珠菌生物膜的效果进行了实验室检测。结果用体积分数2.5%阴香精油作用30 min,对培养3 d的白色念珠菌生物膜达到完全杀灭;作用90 min对培养7 d的白色念珠菌生物膜达到完全清除。用体积分数2.5%阴香精油作用10 min,可完全杀灭悬液内白色念珠菌浮游菌。结论阴香精油对白色念珠菌生物膜有清除效应,可抑制生物膜形成,对悬液内浮游菌杀灭效果更好。

Objective To observe the inhibitory effect of essential oil on Candida albicans biofilm.Methods The modified Brown′s Plate method and the carrier quantitative germicidal test were used to observe the inhibitory effect of essential oil on C.albicans.Results The C.albicans biofilm cultivated for 3 days and 7 days exposed to 2.5%（v/v） essential oil compound preparation for 30 min and 90 min respectively were completely killed.While the pelagic C.albicans in suspensions exposed to 2.5%（v/v） essential oil compound preparation for 10 min were completely killed.Conclusion Essential oil has cleaning effect on C.albicans biofilm and can inhibit biofilm formation.It has better killing effect on pelagic C.albicans.