摘要
Direct chiral separation of azelnidipine enantiomers with Pirkle-type Sumichiral OA-2500 column was studied by normal phase and reserve phase conditions. By normal phase, azelnidipine enantiomers were well separated with Rs as 4.0 using hexane–ethanol （90：10, v/v） as mobile phase, and Rs as 2.7 in shorter time （no more than 13 min） using hexane–ethanol （60：40, v/v） as the recommended mobile phase. They were only partially separated by reverse phase using methanol or methanol containing 0.05 mol/L ammonium acetate. Using same chiral column, the chiral separation of other dihydropyridine calcium antagonist analogues almodipine and nimodipine were also studied and showed partial chiral separation in normal phase.
本文使用Pirkle型Sumichiral OA-2500手性柱,研究了正相和反相色谱直接分离阿折地平对映体的流动相条件。使用正相体系,阿折地平在正己烷–乙醇(90:10,v/v)流动相条件下获得了很好的分离(分离度Rs达到4.0),而使用推荐的流动相正己烷–乙醇(60:40,v/v),则可以在更短的13分钟内获得分离(分离度Rs为2.7)。使用反相的甲醇和含有0.05mol/L醋酸铵的甲醇做流动相,仅能获得部分分离。用同样的实验条件研究了氨氯地平和尼莫地平的拆分,并在正相条件下获得了部分分离。
