GC/MS衍生化测定精浆中游离支链氨基酸和果糖

Determination of free branched chain amino acids and fructose in human seminal plasma as their derivatives by GC/MS

目的建立人精浆中支链氨基酸和果糖的气相质谱衍生化分析方法。方法精液样本液化离心后,按精子活率的临床标准分为正常组和非正常组,经TMS微波衍生化,GC/MS分析两组支链氨基酸、果糖衍生物含量差异。结果正常组与非正常组的缬氨酸,亮氨酸,异亮氨酸在相对含量上均有一定差异。正常组3种氨基酸相对含量高于非正常组。果糖分析观察到同样的特征,正常组相对含量高于非正常组。结论精子活率低的精浆样本仍可检出支链氨基酸和果糖,其差异有助于无精子、少精子精斑的法医学鉴别。

Objective To establish a new method to determine free branched chain amino acids and fructose as their derivatives by GC/MS. Methods After liquefaction and centrifugation, eight seminal samples are divided into two groups, a normal sperm motility group and non-normal sperm motility group, based on clinical diagnosis. The extract from seminal plasma treats with microwave derivatization followed by GC/MS determination. Results The significant difference is shown between the normal group and non-normal group, ineluding higher relative contents of valine, leucine and isoleucine in normal group than those in non-normal group. And higher relative content of fructose is also observed in normal group. Conclusion GC/MS method combining with derivatization can be used to analyze free branched amino acids and fructose in seminal plasma for forensic identification.