百令疏肝胶囊治疗大鼠酒精损伤性肝纤维化的作用

Corbrin shugan capsule for treatment of alcoholic hepatic fibrosis in rats

目的:探讨百令疏肝胶囊对大鼠酒精损伤性肝纤维化的治疗作用。方法:采用酒精反复灌胃,建立大鼠酒精损伤性肝纤维化模型;酶联免疫法测定血清Ⅲ型前胶原肽(ⅢPC)、层粘连蛋白(LN)、基质金属蛋白酶抑制因子-1(TIMP-1)含量;化学比色法测定肝组织羟脯氨酸(Hyp)的含量;肝组织病理切片行Masson三色染色,观察胶原沉积情况,使用Motic Med 6.0数码医学图像分析系统测量肝纤维化面积。结果:百令疏肝胶囊0.09、0.27、和0.45 g.kg-1剂量能明显降低酒精损伤性肝纤维化大鼠血清中TIMP-1、LN水平和肝组织纤维化面积,0.27和0.45 g.kg-1剂量还能明显降低酒精损伤性肝纤维化大鼠血清中ⅢPC水平和肝组织中Hyp含量。结论:百令疏肝胶囊对大鼠酒精损伤性肝纤维化有较好的治疗作用,能降低酒精损伤后大鼠血清中ⅢPC、TIMP-1、LN水平和肝组织中Hyp含量和纤维化面积的增高。

Objective： To investigate the therapeutic effect of Corbrin shugan capsule for treatment of alcoholic hepatic fibrosis in rats.Methods： The rat model of alcoholic hepatic fibrosis was induced by intragastric administration of alcohol repeatedly.The serum procollagen Ⅲ（PC Ⅲ）,laminin（LN） and tissue inhibitors of metalloproteinase-1（TIMP-1） levels were measured with ELISA,and the content of hydroxyproline（Hyp） in liver tissue were determined with colorimetric method.Collagen deposition in liver tissue was observed with Masson′s staining,and the fibrosis area was measured with digital medical image analysis system（Motic Med 6.0）.Results： Compared with the model control group,the serum TIMP-1 and LN levels and hepatic fibrosis area in liver tissue significantly decreased in Corbrin shugan capsule groups with doses of 0.09,0.27 and 0.45 g·kg-1,and the serum PC Ⅲ and the Hyp contents in liver tissue also decreased of Corbrin shugan capsule groups with doses of 0.27 and 0.45g·kg-1.Conclusion： Corbrin shugan capsule can decrease serum PC Ⅲ,TIMP-1 and LN levels and Hyp levels in liver tissue and hepatic fibrosis area in rats,indicating it may have therapeutic effect on alcoholic hepatic fibrosis.