摘要
目的:建立高效液相色谱(HPLC)外标法快速测定人血清中苯巴比妥的浓度。方法:血清样品经甲醇沉淀后,以甲醇-水(40∶60,V∶V)为流动相,流速为1.0 mL·min-1,色谱柱为Inertsil ODS-SP(150 mm×4.6 mm,5μm),柱温为30℃,紫外检测波长为214 nm。结果:血清内源性杂质不干扰待测物的测定,苯巴比妥的线性范围为4.5～72.8μg·mL-1,日内、日间精密度(RSD)均<5%,样品稳定性良好。结论:该法灵敏、快速、准确、操作简便、线性范围宽,可用于苯巴比妥的血药浓度监测。
ABSTRACT OBJECTIVE：To establish a HPLC external standard method human serum. METHODS： Serum samples were precipitated by methanol for rapid determination of Phenobarbital in followed by separation on the column of Inertsil ODS-SP （ 150 mm x4. 6 mm,5 I＆m） at 30 ℃. The mobile phase consisted of methanol-water （40： 60,V： V） at a flow rate of 1.0 mL. min-l. The detecting wavelength was set at 214 nm. RESULTS： The intrinsic impurity of plasma did not interfere with the determination of analyte. The linear range of arbamazepine was 4. 5-72. 8 μg mL^-1. The inter-day and intra-day precisions were less than 5%. The samples showed good stability. CONCLUSION： The established HPLC external standard method is sensitive, fast, accurate and simple with wide linear range and thus it can be applied to the monitoring of serum concentration of Phenobarbital.
出处
《中国医院用药评价与分析》
2012年第9期811-813,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
