川芎嗪对再灌注大鼠心肌细胞c-fos基因表达及凋亡的影响

Effects of tetramethylpyrazine on c-fos expression and apoptosis of myocardial cells in myocardial ischemic reperfusion in rats

目的 :观察川芎嗪 (TMP)对心肌缺血再灌注 (MIR)大鼠心肌 c- fos基因表达及凋亡细胞的作用。方法 :应用免疫组化法检测 MIR不同时期心肌 FOS蛋白的表达 ,并采用末端标记技术对心肌细胞凋亡数进行检测。结果 :1对照组心肌无 FOS蛋白表达 ,亦未见凋亡细胞出现。 2与 MIR组相比 ,TMP干预组心肌 FOS蛋白的表达明显减弱 (6 0 min分别为 5 7± 44个 /片和 6 .4± 3.2个 /片 ,90 m in分别为 46 7± 45 0个 /片和 171± 16 0个 /片 ,12 0 min分别为 2 46± 16 0个 /片和 130± 12 1个 /片 ,均 P<0 .0 5 ) ,TUNEL阳性细胞数均明显减少 (6 0 m in分别为 36 .3± 8.7个 /片和 2 4.7± 7.2个 /片 ,P<0 .0 5 ;12 0 m in分别为 48.0± 2 3.8个 /片和 10 .0± 8.1个 /片 ,P<0 .0 1)。结论 :MIR可诱导终末分化的心肌细胞高表达 FOS蛋白及发生细胞凋亡 ;应用 TMP可使缺血心肌细胞 c-

AIM:To study the effects of tetramethylpyrazine (TMP) on c fos expression and apoptosis of myocardial cells in myocardial ischemic reperfusion (MIR) in rats. METHODS:Coloring TDT mediated dutp nick end labeling (TUNEL) and immunohistochemical staining were used. RESULTS:①No blue apoptosis cells and FOS protein expression were found in non ischemic myocardial tissues; ②Compared with those of MIR rats, the number of FOS immunoreactive positive cells in rats treated with TMP decreased significantly (from 57±44/section to 6.4±3.2/section, 467±450/section to 171±161/section and 246±160/section to 130±121/section respectively, P <0.05 ) in 60, 90 and 120 min and the number of TUNEL positive cells in rats treated with TMP also decreased significantly ( from 36.3±8.7/section to 24.7±7.2/section and 48.0±23.8/section to 10.0±8.1/section respectively, P <0.01) in 60 minutes and 120 minutes. CONCLUSION:Myocardial cells may induce apoptosis in MIR and apoptosis may be related to the increased c fos expression. Administration of TMP can decrease the expression of c fos and the apoptosis of myocardial cells in MIR.