交联剂对复合生物海绵性能的影响

The Influences of Cross-Linking Agent on the Composite Bio-Sponge

目的考察交联剂京尼平和戊二醛对复合生物海绵性能的影响。方法采用冷冻干燥法制得复合生物海绵,采用三硝基苯磺酸吸光光度法测交联度;采用MTT法测细胞毒性;采用溶菌酶降解法测体外降解率。结果①复合生物海绵的交联度均随交联时间的延长而增加,用京尼平和戊二醛交联三天后,复合生物海绵的交联度分别为26.43%和54.63%。②京尼平交联的复合生物海绵吸水性优于戊二醛交联的复合生物海绵。⑧用两种交联剂制得的复合生物海绵的浸提液在细胞培养初期均表现出对细胞增殖的抑制作用,这种抑制作用随培育时间的延长而降低,但戊二醛交联的复合生物海绵其细胞毒性明显高于京尼平。④京尼平或戊二醛交联的复合生物海绵在生理盐水中浸泡4周后,降解率分别为32.1%和28.4%;而在含溶菌酶的生理盐水中浸泡40h后,降解率分别为36.7%和31.2%。结论与用戊二醛交联的复合生物海绵相比,京尼平交联的复合生物海绵交联度降低,吸水性增加,体外降解率增加,细胞毒性明显降低。

Objective To investigate the influence of cross-linking agents genipin and glutaraldehyde on the composite bio-sponge. Methods The composite bio-sponge was prepared with the technology of lyophillization. The degree of cross linking was determined using absorptiometry of trinitrobenzensulfonic acid; the cytotoxicity was tested by MTT assay; the degradation rate in vitro was valuated by lysozyme degradation. Results ①The degree of cross linking of composite bio-sponge crosslinked using genipin and glutaraldehyde increased with the crosslinking time, and reached 26.43% and ,54.63% respectively after crosslinking 3 d.② The water absorption rate of composite bio-sponge crosslinked using genipin was better than that of crosslinked using glutaraldehyde. ③ In the initial stage of cells incubation, all extracts of composite bio-sponges crosslinked using genipin and glutaraldehyde inhibited the growth of the cells, and the inhibition decreased with the incubation time; but the cytotoxicity of composite bio-sponge cresslinked using glutaraldehyde was higher than that of crosslinked using genipin. ④ After soaking in saline for 4 weeks, the degradation rate of composite bio-sponge cresslinked using genipin or glutaraldehyde was 32.1%和28.4%, respectively; however, after soaking in saline containing lysozyme for 40 h, the degradation rate of composite bio-sponge was 36.7%和 %31.2%, respectively.. Conclusion Compared with the composite bio-sponge crosslinked using glutaraldehyde, the degree of cross linking and the cytotoxicity of the composite bio-sponge crosslinked using genipin decreased; however, the water absorption rate and the degradation rate increased.